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Effect of Supplementation on Somatic Parameters
Kostikidou, Marianthi ; Kinkorová, Ivana (advisor) ; Heller, Jan (referee)
Author: Bc. Marianthi Kostikidou Title: Effect of supplementation on somatic parameters Objectives: The aim of this study is to evaluate the effect of a 60-day long supplementation with Tribulus Terrestris on the body composition of healthy men. Methods: This diploma thesis is a double-blinded experiment. The theoretical part was mainly processed on the basis of information from electronic sources in English and Czech language. The subjects of this study were within the range of 18-35 years old and were randomly divided into an experimental (TT1) and a control (TT2) group. We examined changes in basic anthropometric parameters (height, weight, circumferences, skinfolds) and body composition estimated by bioelectrical impedance (BIA). The statistical analysis of the data was evaluated using ANOVA. The level of significance was set to 0.05. Results: In TT1 we observed significant changes in anthropometric parameters. Changes in body composition estimated by BIA were not of statistical significance. Statistically significant changes in TT2 were noted in both anthropometric measurements and BIA. Changes in both groups could be caused by various factors. Keywords: ergogens, nutritional supplements, tribulus terrestris, testosterone, caliperation, body composition, bioelectrical impedance
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Comparison of the determination of hormones (Follicle stimulating hormone, Luteinizing hormone, Prolactin, Testosterone, Progesteron) by two analytical systems. Converting accredited method and its verification.
Kucejová, Soňa ; Martínková, Markéta (advisor) ; Mrízová, Iveta (referee)
Analytical system ARCHITECT i2000SR was verified according to requirements of ÚLBLD VFN and 1. LF UK laboratory in Prague. Repeatability, intermediate precision, and measurement uncertainty were determined as performance parameters for verification of analytical assays for testosterone, progesterone, luteinizing hormone, follicule stimulating hormone and prolactin. Results of Lyphochek control samples, which were measured, were consistent with values given by manufacture. Repeatability: coefficients of variation for testosterone Lyphochek 1 6,81%, for Lyphochek 3 6,40%, progesterone 2,4% and 1,8%, luteinizing hormone 5,38% and 1,89%, follicle stimulating hormone 5,12% and 3,24% prolactin 1,45% a 1,83%. Intermediate precision: coefficients of variation for testosterone Lyphochek 1 6,02%, Lyphochek 2 3,60%, Lyphochek 3 3,07%, progesterone 7,9%, 4,9% and 5,8%, luteinizing hormone 4,50%, 5,51% and 5,83%, follicle stimulating hormone 4,00%, 3,72% and 4,87%, prolactin 4,60%, 4,20% and 5,00%. Measurement uncertainty: testosterone 6,02%, progesterone 7,9%, luteinizing hormone 5,83%, follicle stimulating hormone 4,87%, prolactin 5,00%. Analytical System Architect i2000SR was compared with previously used ADVIA Centaur system to find out, whether it is possible to convert the method Centaur Testosterone,...
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Hormonal control of male sexual behaviour in reptiles
Schořálková, Tereza ; Kratochvíl, Lukáš (advisor) ; Landová, Eva (referee)
The most important hormone affecting typical male sexual behavior in reptiles is testosterone, but also other steroid hormones such as dihydrotestosterone, estrogens or progestins may be involved in this regulation. According to the organizational-activational hypothesis testosterone acting during early ontogenic stage has an organizational effect on adult behavior and this behavior is triggered by influence of recent levels of hormones. Moreover, it was formulated the hypothesis on temporary organization of male sexual behvior that could change existing interpretations. Reptile reproductive strategies use to be classified according to gametogenesis and steroidogenesis as associated or dissociated reproductive strategies howerever, this division is rather artificial. This review summarizes existing knowledge of the hormonal influence of sexual behavior in reptiles, and comments the most common methods.
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Biological and related theories of aggression and its humoral regulation
Bradáč, Ondřej ; Komárek, Stanislav (advisor) ; Havlíček, Jan (referee)
Aggression is a phenomenon which acquired a substantial share on attention of biological literature, especially since the beginning of 20th century. In this thesis, various approaches to the problem from the field of biology are summarized. Intensified attention is dedicated to the theoretical concepts of aggression, especially the concepts consulting the problem of aggression from the evolutionary point of view and to the research on the field of hormonal regulation of aggression, stressing out the role of the hormone testosterone, and to particular hypothesis, which take account on its mutual relationship with behavior. The empirical part of the thesis is dedicated to verification of premises of the challenge hypothesis according to the relationship between testosterone and aggressive behavior. Significant findings of the empirical part refer to the existence of a link between basal level of testosterone and aggressive tendencies and to the existence of a negative link between basal level of testosterone and its increase in reaction to a competitive situation.
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Noninvasive measurement of steroid homones and effect of hormonal manipulation on behaviour in the gecko Paroedura picta
Matušková, Lucie ; Kratochvíl, Lukáš (advisor) ; Fraňková, Marcela (referee)
Hormones influence life of all animals. Not only they affect physiological changes in organisms, but also impact their behaviour. This work focuses at two main groups of steroid hormones: glucocorticoids and androgens. Glucocortiods are activated in response to stress. Their levels can be measured using non-invasive methods, which have a range of advantages. The main advantage is the feedback-free sample collection for enzyme immunoassay. As the measurement involves metabolites of the hormones rather than the hormones themselves, prior validation of the method is, however, necessary. This work reports on a study aiming to validate non-invasive measurement on the Madagascar Ground Gecko (Paroedura Picta). The validation was based on ACTH challenge test: Synacthen Depot was injected, which should lead to increased blood level of glucocorticoids. The validation, however, was not successful. The measurement did not discover significant increase in the levels of the metabolites of glucocorticoids. In addition, the work focuses on behavioural effects of testosterone, the primary androgen. Hormonal manipulations have been carried out on several male and female specimens. The results have discovered differences in sexual behaviour between control groups. On the other hand, the hormonal manipulations had no...
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Hormonal control of male traits in leopard gecko, Eublepharis macularius
Schořálková, Tereza ; Kratochvíl, Lukáš (advisor) ; Rehák, Ivan (referee)
It is generally believed that the presence of male traits is regulated by androgens. This thesis is designed to reveal the influence of testosterone on male traits in the leopard gecko, Eublepharis macularius. The noninvasive skin application mixture of vegetable oil and testosterone was used to manipulate the hormonal status of females and castrated males. This method has proven to be an effective alternative to the widely used hormonal implants. Manipulation was successfully validated, and there was apparent progressive increase of male sexual behavior in both groups. In females, the onset lasted much longer time, but both groups were able to perform all components of males sexual behaviour. Furthermore, I found that, contrary to widespread view, male sexual behavior persisted several weeks after reduction of testosterone levels in both manipulated groups. These results bring new evidence for temporary organization of male sexual behaviour by testosterone. Powered by TCPDF (www.tcpdf.org)
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Mechanisms of relationship between melanin-based colouration and behavioural syndromes in birds
Nacházelová, Martina ; Javůrková, Veronika (advisor) ; Tomášek, Oldřich (referee)
Melanins are the most common pigments incorporated into birds' feathers. Since melanin-based coloration is variable in the extent and intensity, its function is considered to be particularly cryptic and mechanical, hardening the feather structure. However, recently it has been found that melanin- based coloration also has a signaling function, due to the observed correlation between this type of coloration and many physiological and behavioral traits. These relationships follow from the pleiotropic effects of genes regulating the melanocortin system and the physiological effects of testosterone and corticosterone, which are involved in melanogenesis. This work describes genetic and physiological mechanisms of the relationship between melanin-based coloration and behavioral traits in birds and, based on available studies, supports the signaling function of this type of coloration. Melanin-based coloration has been shown to be positively correlated with aggressiveness and dominance, sexual activity, coping with stress, a fast strategy of exploratory behavior, parental care, and anti-predator behavior in many bird species. However, despite intense research interest in this topic in last decade, it is worth noting that more experiments focused on species with a distinct sexual dichromatism or...
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Changes in Hormone Levels in Female Basketball Players and Their Relationship with Emotional state and Efficiency
Zikánová, Tereza ; Pivoňková, Věra (advisor) ; Hroníková, Linda (referee)
This thesis focuses on women's competition in female basketball players and their hormonal responses to winning and losing. We tested saliva samples of 29 female players for testosterone and cortisol which were collected in three phases: baseline samples, pregame samples and after game samples. In every collection phase we also inquired about the emotional state of mind of the respondents. Players completed a survey examining their subjective evaluation of the match result. We also used statistical data from every match, which provided an objective evidence on the players' usefulness. All the aforementioned variables were examined in relation to the testosterone and cortisol levels. The results have revealed that the cortisol levels are responsive to the match results, whereas the losing players display a significant rise of the stress hormone level after the match. The winners display only a slight increase in the cortisol levels. Testosterone levels did not prove to react to the match results. However, it was discovered that the winners exhibited higher levels of testosterone in all collection phases than their competitors. Nevertheless, the difference between the winners and the losers was not significant in any of the cases. The winners felt more positive after the match than the losers. The correlation...
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Hormones and Competitive Behavior
Sýkora, Zdeněk ; Cingl, Lubomír (advisor) ; Bauer, Michal (referee)
The thesis aims to contribute to the literature on the biological underpinnings of important economic behaviors. Financial markets can become dangerously unstable from many reasons such as the hormones contained in our bodies. We primarily focus on the effect of basal testosterone and cortisol on willingness to compete and risk taking. We also investigate their interaction called the dual-hormone hypothesis, because it has not been sufficiently analyzed and replicated so far. We run a controlled laboratory experiment with 96 university students and have gender-balanced dataset, thus we can also examine gender differences. We find no support that higher testosterone increase willingness to compete in men as opposed to most of the recent literature. Moreover, higher levels of testosterone decrease competitiveness but only for women. We further find positive effect of the 2D:4D ratio for women and negative effect of trait anxiety for men on competitiveness. There are also substantial gender differences in competitive and risk taking behavior. We cannot confirm the dual-hormone hypothesis for willingness to compete. But we find significant support for the dual-hormone hypothesis for risk taking for women and with negative effect of testosterone on risk taking. The effects stay robust even after...
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